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KMID : 1234420080360040326
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 4 p.326 ~ p.335
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Structural Identification of SiderophoreAH18 from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper
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Woo Sang-Min
Kim Sang-Dal
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Abstract
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The siderophore (siderophoreAH18) of Bacillus subtilis AH18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LH-20 chromatography, and reversed-phase HPLC. The siderophoreAH18 was identified bacillibactin with its structure by GC-MS, 1H-NMR, and 13C-NMR. SiderophoreAH18 (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of (2,3-dihydroxybenzoate-glycine-threonine)3. Purified siderophoreAH18 showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.
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KEYWORD
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Bacillibactin, PGPR (plant growth promoting rhizobacterium), siderophore, Bacillus subtilis AH18
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